National Under-23 coach Conti Kubheka is optimistic that Amagluglug can emulate the class of 2000 and qualify for a second straight Olympic Games.
The South Africans tackle Zambia on Saturday in Potchefstroom and a win is vital in order to keep pace with group leaders Ghana.
Algeria are the fourth team in the group and were thrashed 4-1 by the South Africans in Rustenburg earlier this month.
South Africa opened their campaign with a high-controversial 1-0 loss to Ghana in Accra a few months back.
Kubheka, however, told the SuperSoccer website that he is not underestimating the Zambians.
"It will be a tough one because Zambia are still to win a match since the start of the qualifying matches in October," Kubheka is quoted to have said.
"Everything is fine here in Potchefstroom and we are preparing well for Zambia. It's good that we know what to expect from Zambia after seeing them in action during the All Africa Games finals in Abuja in October.
"Zambia are a very good side but we won't allow them to register their first win of the tournament at our expense. They are in big trouble because we have managed to secure all our top players for Saturday's game this time around, he added.
"The only disappointment is that Benedict Vilakazi will not be available for this match after collecting his second yellow card during our last match against Algeria in Rustenburg at the beginning of this month.
"However, we have many gifted youngsters who will do the job for the country. I would like to urge all the people of Potchefstroom and surrounding areas to come in their big numbers and support us to send Zambia back home empty-handed.
"It will be tough but there is no doubt that we will qualify for the finals in Athens next year," said Kubheka, who has called up four new players to the Bafana Bafana feeder team.
Mabuti Khanyeza, Siphesihle Ngobese, Papi Zotwane and Bevan Fransman were called-up by Kubheka to bolster the squad.