A 90th minute goal from Peter Khobane earned SuperSport United all three points and the bragging rights in the city of Pretoria after they beat Sundowns 1-0 in a Castle Premiership game played at the Odi Stadium on Wednesday night.
The first round game between the two clubs ended in a 1-1 draw.
Matsatsantsa coach Pitso Mosimane was visibly ecstatic after the game during his interview on SuperSport 3.
"The game is my life", he said. "There is nothing better than beating Sundowns. Our team might not be as big as them, but this result shows that we have arrived on the scene."
The first half produced relatively little in the way of excitement and goal-scoring opportunities with neither side being able to get a grip on the game.
SuperSport did however, go straight onto the attack and came close to scoring in the opening 40 seconds following an attack down the left hand flank, but McDonald Mukhansi was not able to make sufficient contact with the ball and Sipho Nunens was able to scramble the ball clear with Down's keeper Andre Arendse looking in trouble.
In the 14th minute Sashi Chalwe was inches away from opening the scoring when his powerfully struck free-kick from 30-yards out flew just over Calvin Marlin's crossbar,
In the 29th minute Mandla Zwane played a delightful ball through to Abram Raselemane, whose first time volley brought out the best in Arendse.
In the 31st minute the Brazilians were momentarily reduced to nine men when both Michael Manzini and Lucas Sebona left the field after receiving head wounds in the hotly contested game.
Manzini, who was taken to hospital, was replaced by Craig Bianchi while Sebona was able to resume his duties after some treatment.
In the 41st minute Simba Maruma was desperately unlucky to see his powerful header from an Ishmael Maluleke cross canon off the inside of the upright and back into play with United keeper Marlin well beaten.
Raselemane had a late chance from range, but his low shot was comfortably dealt with by Arendse.
Substitute Daniel Matsau should have put Matsatsantsa into the lead in the 57th minute with his first touch following a great defence splitting ball from Mukhansi, but he blasted over the bar.
A minute later Sebona slid in to meet an Isaac Shai cross, but his effort hit the crossbar from close range.
The rebound fell to Rotson Kilambe, who was unlucky to see his goal-bound shot bounce back off defender Ricardo Katza.
In the 70th minute Kilambe did well to get to the by-line and pull the ball back across the goal, but Marumo arrived just to late to apply the finishing touch.
In the 79th minute a loose ball fell to Mandla Zwane on the edge of the area and the defender volleyed the ball first time towards goal. His shot dipped viciously towards the top corner, but Arendse did brilliantly to tip the ball to safety.
A few moments later Matsau also came close with a well struck shot from outside the box, but the ball flew narrowly wide of the target.
The game seemed to be drifting to a draw, but then in the 90th minute Zwane delivered a quality ball into the back post and substitute Peter Khobane was on hand to power home his header to give Pitso Mosimane's men all three points and the mantle of 'Kings of soccer in Pretoria'.